Understanding NKT cells through sulfatide analogues

Abstract The goal of this research is to further investigate NKT cell activation using sulfatide analogues. cells have been shown play a significant role in mediating inflammatory immune responses and because this, there has strong interest pharmacologically regulating compartment fight cancer. recognize lipids that are loaded on CD1d molecules the surface cells, it structural modifications these directly affect polarization. Our work focused impact sulfatide, which glycolipid can be onto known activate sulfatide-reactive (generally type II) when presented by monomers plastic. We numerous analogues with (based previous alpha-galactosylceramide analogues) acyl chain, sphingosine changes beta-anomeric alpha-anomeric. These screened hybridomas, DN32 XV19, assess their effect I II respectively. observed alterations or chain sulfatides do not always mimic effects activity similar (not sulfatide-reactive) monomer bone marrow-derived dendritic cells. results provide insight into identified may applicable cancer therapy activating NCI, NIH intramural program (NIA-C-004020), institutional grants (R01 GM111849)